The filamentous fungus Trichoderma reesei is extensively used in the biofuel industry due to its remarkable ability to deconstruct lignocellulosic biomass, a critical process in bioethanol production. This fungus employs various sugar transporters, such as Tr44175 and Tr69957, to facilitate the uptake of key carbohydrates like cellobiose and sophorose, which are essential for regulating the expression of cellulolytic enzymes and thereby enhancing cellulose hydrolysis. Moreover, studies on membrane proteins with CFEM domains, such as Tr111205, have shown that they not only improve sugar uptake but also play roles in osmotic stress response and cell wall integrity. Molecular docking analyses indicate that these proteins have a high specificity for binding sugars, optimizing metabolic efficiency across diverse lignocellulosic substrates, including sugarcane bagasse. These findings expand our knowledge of nutrient sensing in T. reesei and suggest strategies for strain engineering to boost enzyme production, which could significantly reduce costs in industrial bioprocessing.