Actinomycetes are a heterogenous group of Gram-positive bacteria belonging to the phylum Actinomycetota. They constitute one of the largest bacterial phyla and are present in terrestrial and aquatic ecosystems, mainly in soil. Some genera such as Streptomyces are well known as a rich source for novel antibiotics, insecticides and other secondary metabolites that could be useful in medicine, biotechnology, and agriculture, which makes them valuable bacteria for research and applications. However, the phylum also includes pathogenic species, the most well-known is Mycobacterium tuberculosis, the causative agent of tuberculosis. Other species can induce diseases in humans and animals such as Nocardia brasiliensis or Actinomadura madurae, which causes Nocardiosis and mycetoma (e.g. madura foot). One approach to control these pathogenic agents might be the use of bacteriophages. For rare actinomycetes, bacteriophages have been used as a selective tool to isolate novel natural product producer rather than as potential therapeutic agents. Our aim was to isolate new bacteriophages against rare actinomycetes from the DSMZ collection as an alternative control agent against infections with pathogenic actinomycetes and/or for selective isolation of specific actinomycetes. A set of 22 phages were isolated against seven different strains of actinomycetes notably three against Lentzea rhizosphaerae and one against Actinomadura meyerae. The next steps to fully characterize all the newly-obtained phages are to acquire TEM images for each of them, establish their host range and test their endurance, mainly their stability at different pH and temperatures. Another essential step is to sequence their genome and to analyze their characteristics in relation to their host. This work is currently in progress. Morphological characterisation identified the Lentzea phages as a siphovirus. They present all a narrow host range and preliminary results about their stability at different pH and temperature conditions point to a high stability. Another interesting phage is the Actinomadura meyerae phage vB_AmeS_Stercus. Host range analysis revealed a narrow host range, genomic analysis identified genes of a lysogeny cluster, thus identifying it as a temperate phage. Further experiments are in progress to express the endolysin of vB_AmeS_Stercus as an alternative to fight against Actinomadura pathogenic strains (e.g. A. madurae, A. latina).