Microscopy Conference 2023

Structure of the HOPS tethering complex, a lysosomal membrane fusion machinery

LS 1: High-resolution cryo-EM

In 2020, cryo-EM breached another barrierer and it has now been shown that it can visualise individual atoms within a macromolecular complex. Significant improvements in software and mircoscope design were mandatory for this achievement. However, what is possible for ideal samples or test-specimen must not necessarily be true for research-based biological applications, highlighting the prerequisite of sample quality and optimization which continues to be the major challenge en route to atomic-resolution cryo-EM structures. The session will showcase how to improve the sample handling, microscope setup and image processing to obtain high-resolution structures using cryo-EM.

High resolution cryo-EM

Abstract talk

LS 7: Advances in sample preparation

Design and deployment of a high-performance computing system for cryo-EM

Poster session LS 1: High-resolution cryo-EM

Poster

LS 4: Image analysis of large data sets

Cryo-EM analyses of the conformational spectrum of an ABC transporter in various membrane mimics

Structural basis of TIRAP assembly revealed by cryo-EM

Constructing artificial membrane structures on graphene to resolve membrane protein conformational dynamics

Arne Möller

Einsätze

27.02.2023