Microscopy Conference 2023

Cryo-STEM imaging for single particle structure determination

IM 4: Development of cryo-EM instrumentation and techniques

Technological advances steadily push the limits of cryo-electron microscopy (cryo-EM). In recent years, novel developments in cryo-EM instrumentation as well as newly established workflows enabled groundbreaking insights in both, life sciences and material sciences.
In this session we will discuss new developments in experimental cryogenic techniques, cryo-sample preparation as well as instrumental capabilities applied across different fields.
Contributions are welcome from, but not restricted to, the following areas: SEM, FIB, TEM, STEM and CLEM at cryogenic temperatures, including hardware improvements, cryo-stages, cryogenic sample preparation techniques used for EM, measurements of local temperature as well as image processing for cryo-EM.


Development of cryo-EM instrumentation and techniques

Abstract talk

LS 1: High-resolution cryo-EM

Integrated differential phase contrast (iDPC) STEM for ultimate low dose imaging at atomic resolution, including cryo nano particles

IM 6: Phase-related techniques & 4D STEM

Poster session IM 6: Phase-related techniques & 4D STEM

Poster

Membrane analysis toolkit: a quantitative way to analyse protein lipid mixtures from cryo-EM images

LS 4: Image analysis of large data sets

Poster session LS 4: Image analysis of large data sets

LS 2: Cryo-tomography

Effect of multiple scattering and the ice thickness on the information transfer in cryo transmission electron microscopy

This symposium is hosted by the DGE Young Microscopists working group (yDGE) and will cover topics especially interesting for young researchers. For this, we invited experts who will give insights on publication writing and career planning.
In addition, we want to offer students the opportunity to give a short talk on their work in front of their young colleagues, to gain presentation experience in a casual atmosphere and enhance the scientific exchange.
The newly founded working group DGE Young Microscopists will briefly introduce their activities and possibilities to join.
Our goal is a lively, relaxed exchange between students and early-career postdocs to connect to like-minded colleagues. For this, the symposium will be followed by a social event nearby the conference's venue.


DGE young microscopists symposium

Poster session LS 1: High-resolution cryo-EM

Wirtinger Flow: the effect of different noise models for low-dose scanning electron transmission microscopy

Cryo-EM structures of <em>C. glutamicum</em> pre- and posttranslocational 70S ribosome in complex with Kasugamycin

In 2020, cryo-EM breached another barrierer and it has now been shown that it can visualise individual atoms within a macromolecular complex. Significant improvements in software and mircoscope design were mandatory for this achievement. However, what is possible for ideal samples or test-specimen must not necessarily be true for research-based biological applications, highlighting the prerequisite of sample quality and optimization which continues to be the major challenge en route to atomic-resolution cryo-EM structures. The session will showcase how to improve the sample handling, microscope setup and image processing to obtain high-resolution structures using cryo-EM.

High resolution cryo-EM

Visualisation of native Atg9 complexes using <em>in situ </em>cryo-EM

Poster session LS 2: Cryo-tomography

LS 5: Correlative and multimodal microscopy

ER-C 2.0: An update on plans for new electron microscopes at the Ernst Ruska-Centre in Forschungszentrum Jülich

IM 1: Progress in instrumentation and ultrafast EM

This session will cover recent advancements of instrumentation for imaging and analytical purposes of transmission electron microscopy (TEM and STEM). Part of this session will focus on time-resolved electron microscopy and its various aspects to study optical excitations and nonequilibrium phenomena in materials. Contributions are welcome to describe the state of the art of instrumentation for high resolution imaging as well as all variants of analytical techniques.

Progress in instrumentation and ultrafast EM

Invited talk

spectrum A

The role of electron microscopy in fighting the pandemic

Plenary session

Plenary chair

Cryo-EM, HRTEM, ptychography

poster session 5

Poster presentations

Session chairs

Image analysis is an integral part of the single-particle as well as the tomography cryo-EM workflows. In single-particle cryo-EM, automated image acquisition procedures generate large data sets that enable the ensemble structure determination of increasing number of conformations at higher resolution. This procedure also requires algorithms that can detect the variability in large data sets and distinguish different conformations from each other. Similarly, using subtomogram averaging the analysis of structural variability is driven by improved classification techniques of larger data sets. Due to the processing of volumes instead of projections, this method is more computationally intensive than single particle analysis and therefore requires new software solutions. Moreover, image processing of large numbers of cellular cryotomograms by new segmentation and template matching methods will enable a quantitative image analysis of the cellular environment.

Carsten Sachse

Einsätze

27.02.2023

28.02.2023

01.03.2023