Microscopy Conference 2023

Towards multimodal <em>in situ</em> cryo-FIB-SIMS imaging in biological systems

IM 4: Development of cryo-EM instrumentation and techniques

Technological advances steadily push the limits of cryo-electron microscopy (cryo-EM). In recent years, novel developments in cryo-EM instrumentation as well as newly established workflows enabled groundbreaking insights in both, life sciences and material sciences.
In this session we will discuss new developments in experimental cryogenic techniques, cryo-sample preparation as well as instrumental capabilities applied across different fields.
Contributions are welcome from, but not restricted to, the following areas: SEM, FIB, TEM, STEM and CLEM at cryogenic temperatures, including hardware improvements, cryo-stages, cryogenic sample preparation techniques used for EM, measurements of local temperature as well as image processing for cryo-EM.


Development of cryo-EM instrumentation and techniques

Abstract talk

LS 5: Correlative and multimodal microscopy

Correlative microscopy and imaging secondary ion mass spectrometry for high resolution nano-analytics on biological tissue

Correlative microscopy aims at a combination of different imaging modalities to analyze a single specimen by more than one microscopic technique. Recent developments in microscopy as well as in computational approaches make it more and more feasible to combine different imaging modalities in a correlative and multimodal way. Therefore, this session will focus not only on the ‘classic combination’ of light and electron microscopy but will cover also other approaches that include X-ray, Raman- or atomic force microscopy (AFM). It is also aimed to report on new computational approaches, such as machine learning to analyze the obtained CLEM data.

Correlative and multimodal microscopy

IM 1: Progress in instrumentation and ultrafast EM

aurum

Instrumentation and methods

Tatjana Taubitz

Vortrag

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