Microscopy Conference 2023

Correlative microscopy and imaging secondary ion mass spectrometry for high resolution nano-analytics on biological tissue

LS 5: Correlative and multimodal microscopy

Correlative microscopy aims at a combination of different imaging modalities to analyze a single specimen by more than one microscopic technique. Recent developments in microscopy as well as in computational approaches make it more and more feasible to combine different imaging modalities in a correlative and multimodal way. Therefore, this session will focus not only on the ‘classic combination’ of light and electron microscopy but will cover also other approaches that include X-ray, Raman- or atomic force microscopy (AFM). It is also aimed to report on new computational approaches, such as machine learning to analyze the obtained CLEM data.

Correlative and multimodal microscopy

Abstract talk

IM 1: Progress in instrumentation and ultrafast EM

Towards multimodal <em>in situ</em> cryo-FIB-SIMS imaging in biological systems

IM 4: Development of cryo-EM instrumentation and techniques

Technological advances steadily push the limits of cryo-electron microscopy (cryo-EM). In recent years, novel developments in cryo-EM instrumentation as well as newly established workflows enabled groundbreaking insights in both, life sciences and material sciences.
In this session we will discuss new developments in experimental cryogenic techniques, cryo-sample preparation as well as instrumental capabilities applied across different fields.
Contributions are welcome from, but not restricted to, the following areas: SEM, FIB, TEM, STEM and CLEM at cryogenic temperatures, including hardware improvements, cryo-stages, cryogenic sample preparation techniques used for EM, measurements of local temperature as well as image processing for cryo-EM.


Development of cryo-EM instrumentation and techniques

aurum

Instrumentation and methods

Tatjana Taubitz

Vortrag

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