Microscopy Conference 2023

Biochemical and structural studies of NAPDH oxidase

LSLB: Late breaking abstracts

Late breaking posters LS

Poster

LS 1: High-resolution cryo-EM

Cryo-EM structure of the <em>E. coli</em> formate hydrogenlyase complex

Understanding redox protein mechanism through structure

In 2020, cryo-EM breached another barrierer and it has now been shown that it can visualise individual atoms within a macromolecular complex. Significant improvements in software and mircoscope design were mandatory for this achievement. However, what is possible for ideal samples or test-specimen must not necessarily be true for research-based biological applications, highlighting the prerequisite of sample quality and optimization which continues to be the major challenge en route to atomic-resolution cryo-EM structures. The session will showcase how to improve the sample handling, microscope setup and image processing to obtain high-resolution structures using cryo-EM.

Bonnie J. Murphy

Vortrag

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