An Staes (Zwijnaarde / BE), Teresa Maia (Zwijnaarde / BE), Sara Dufour (Zwijnaarde / BE), Robbin Bouwmeester (Zwijnaarde / BE), Ralf Gabriels (Zwijnaarde / BE), Lennart Martens (Zwijnaarde / BE), Kris Gevaert (Zwijnaarde / BE), Francis Impens (Zwijnaarde / BE), Simon Devos (Zwijnaarde / BE)
Data-independent acquisition (DIA) has become a well-established method for MS-based proteomics. However, the list of options to analyze this type of data is quite extensive, and the use of spectral libraries has become an important factor in DIA data analysis. More specifically the use of in silico predicted libraries is gaining more interest. By working with a differential spike-in of human standard proteins (UPS2) in a constant yeast tryptic digest background, we evaluated the sensitivity, precision and accuracy of the use of in silico predicted libraries in DIA data analysis workflows compared to more established workflows. Three commonly used DIA software tools, DIA-NN, EncyclopeDIA and SpectronautTM, were each tested in spectral library mode and spectral library-free mode. In spectral library mode, we used independent spectral library prediction tools PROSIT and MS2PIP together with DeepLC, next to classical data-dependent acquisition (DDA)-based spectral libraries. In total, we benchmarked twelve computational workflows for DIA and one for DDA. Our comparison showed that DIA-NN reached the highest sensitivity while maintaining a good compromise on the reproducibility and accuracy levels in either library-free mode or using in silico predicted libraries pointing to a general benefit in using in silico predicted libraries.