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  • P-I-0076

PhyloFunc: phylogeny-informed functional distance as a new ecological metric for metaproteomic data analysis

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Microbiology and Microbiome Analysis

Poster

PhyloFunc: phylogeny-informed functional distance as a new ecological metric for metaproteomic data analysis

Thema

  • Microbiology and Microbiome Analysis

Mitwirkende

Luman Wang (Beijing / CN), Caitlin M.A. Simopoulos (Ottawa / CA), Joeselle M. Serrana (Ottawa / CA), Zhibin Ning (Ottawa / CA), Boyan Sun (Beijing / CN), Jinhui Yuan (Beijing / CN), Daniel Figeys (Ottawa / CA), Leyuan Li (Beijing / CN)

Abstract

Luman Wang1, Caitlin M. A. Simopoulos2, Joeselle M. Serrana2, Zhibin Ning2, Boyan Sun3, Jinhui Yuan3, Daniel Figeys2,*, and Leyuan Li3,*

1 Department of Health Informatics and Management, School of Health Humanities, Peking University, Beijing 100191, China

2 School of Pharmaceutical Sciences, and the Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, K1H 8M5, Canada

3 State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, China

* Corresponding authors: L.L.: lileyuan@ncpsb.org.cn; D.F.: dfigeys@uottawa.ca

Background

Beta-diversity is a fundamental ecological metric for exploring dissimilarities between microbial communities. On the functional dimension, metaproteomics data can be used to quantify beta-diversity to understand how microbial community functional profiles vary under different environmental conditions. Conventional approaches to metaproteomic functional beta diversity often treat protein functions as independent features, ignoring the evolutionary relationships among microbial taxa from which different proteins originate. A more informative functional distance metric that incorporates evolutionary relatedness is needed to better understand microbiome functional dissimilarities.

Results

Here, we introduce PhyloFunc, a novel functional beta-diversity metric that incorporates microbiome phylogeny to inform on metaproteomic functional distance. Leveraging the phylogenetic framework of weighted UniFrac distance, PhyloFunc innovatively utilizes branch lengths to weigh between-sample functional distances for each taxon, rather than differences in taxonomic abundance as in weighted UniFrac. Proof-of-concept using a simulated toy dataset and a real dataset from mouse inoculated with a synthetic gut microbiome and fed different diets show that PhyloFunc successfully captured functional compensatory effects between phylogenetically related taxa. We further tested a third dataset of complex human gut microbiomes treated with five different drugs to compare PhyloFunc"s performance with other traditional distance methods. PCoA and machine learning-based classification algorithms revealed higher sensitivity of PhyloFunc in microbiome responses to paracetamol.

Conclusions

Unlike traditional approaches that consider metaproteomics features as independent and unrelated, PhyloFunc acknowledges the role of phylogenetic context in shaping the functional landscape in metaproteomes. In particular, we report that PhyloFunc accounts for the functional compensatory effect of taxonomically related species. It is effective, ecologically significant, and has better sensitivity, as evidenced by the particular applications we presented.

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