Reactome biomolecular pathways database – update

The Reactome database of curated human pathways (Milacic et al, 2024) covers 11,442 proteins in 15,212 reactions supported by 38,549 literature references (Release 88, March 2024). Building on our successful collaboration with the DiseaseMaps community (Ostaszewski et al, 2021), we are increasing our efforts in pandemic preparedness, among others through the recent release of the Respiratory Syncytial Virus (RSV) Infection Pathway. We are now also providing "early access" to pathways still awaiting external review, to extend the available data, and to invite community collaboration around specific curation topics.

Pathway analysis capabilities range from simple gene enrichment to quantitative multi-omics analysis with ReactomeGSA (Griss et al. 2020). Analysis can be submitted through an R interface, and results are presented interactively in a visually attractive genome-wide results overview based on Voronoi maps. ReactomeGSA now includes a function that enables users to directly search for public datasets in Expression Atlas (Moreno et al., 2022) and GREIN (Mahi et al., 2019) from within ReactomeGSA, that can then be loaded into a ReactomeGSA analysis. These new features are available through a completely re-developed web interface as well as the ReactomeGSA Bioconductor R package. In the updated ReactomeGSA we are also piloting a new look-and-feel, and integrated user guidance, features planned for the next version of the Reactome home page, which will be released in 2025.

Reactome is open source, open data, open graphics, all components are distributed under permissive licenses (CC0, CC-BY).