Obesity is characterized by the excessive accumulation of body fat. And this is recognized as a leading risk factor for various chronic diseases, including cardiovascular diseases, type 2 diabetes, certain cancers, respiratory disorders and etc. Gamma-aminobutryic acid (GABA) diet is considered to have anti-obesity effect. In this study, we performed proteomic profiling of liver in obese mice non-treated or treated with GABA. High-fat diet-induced male C57BL/6J mice, 18 week old, were randomly allocated to two groups; administrated without, or with oral GABA at 14.3 mg/kg/day for 5 weeks. At the end of experiment, mice were euthanized using CO2, and then tissue samples were collected. We performed proteomic analysis using liquid chromatography and tandem mass spectrometry (LC–MS/MS) analysis. Proteomic profiling revealed 136 proteins statistically significant changes (p-value < 0.05, fold-change > 2); 54 up-regulated and 82 down-regulated for GABA-treated mice. Also we performed analysis for gene ontology (GO, p-value < 0.05) to assess the effect of obesity. The GO analysis for biological processes showed that differentially expressed proteins (DEPs) participated in metabolic process and catabolic process. And the GO terms for molecular function indicated that DEPs mainly enriched in the binding and activity.