Part II
Keywords:
Volume EM, Life Sciences, sample preparation, focused ion beam scanning electron microscopy, serial block-face scanning electron microscopy, room temperature, targeting
Abstract:
Motivation:
In this workshop, we will cover common volume EM techniques (FIBSEM, SBEM, AT), with a special focus on sample preparation tips and tricks, and an outlook for data analysis. The workshop is tailored for people starting vEM projects as well as more experienced users. We will give examples of workflows and recurrent challenges, and offer a discussion forum on sample preparation protocols. The workshop is planned in an interactive format with round table discussions and networking opportunities.
Contents:
· Overview of common vEM modalities in Life Sciences
· Commonly used sample preparation protocols
· How to target your volume of interest (Xray, CLEM, …)
· Outlook on data analysis workflows and hurdles
· Time to ask your questions/discuss your problems