Abstract text (incl. figure legends and references)
The centriole (also called basal body) is an evolutionary conserved organelle that coordinates fundamental biological processes including cell division, cellular signaling, and cell motility. This organelle, 500 nm long and 250 nm in diameter, is composed of about 100 different proteins, some of which have been associated with human diseases. How these proteins are organized at the level of the centriole architecture and how associated mutations could be involved in pathologies is still poorly understood. I will present the latest work from my laboratory, which tackle these fundamental structural cell biology questions using cryo-tomography, cell biology, and ultrastructure expansion microscopy (U-ExM) methods.