Abstract text (incl. figure legends and references)
Redox proteins carry out sophisticated reactions that shape our bodies, our planets and our climate. Understanding the mechanism of redox proteins depends on an atomic-level understanding not only of the protein structure, but of structural changes associated with reduction and oxidation processes. Because structural studies by single-particle cryo-EM have only recently achieved the resolutions that allow for atomic-level insight into mechanism, the tools for preparation of samples under defined redox conditions are lacking. As our group has worked toward understanding the structure and function of redox proteins, we have brought together a set of tools for better assessing redox-dependent structural changes. Applying these tools to proteins involved in hydrogen production and methanogenesis have allowed us to gain new insight into these processes.