Microscopy Conference 2023

Focus on the 3D architecture of mitotic spindles in human cells

LS 3: Imaging of large volumes and plastic section tomography

Throughout the last two decades different volume scanning electron microscopy (vEM) methods have gained more importance in the life sciences allowing new questions to be answered for samples from cells to organisms and tissues. New developments for hardware and software for transmission as well as scanning electron microscopy have paved the road to new exciting research topics to be addressed. This session will illustrate the diversity of methods and applications of the current volume SEM modalities, namely array tomography (AT), serial block face SEM (SBEM) and focused ion beam SEM (FIB-SEM), as well as plastic tomography focusing on higher resolution. Besides different research topics, the challenges linked to the sample preparation, imaging and data analysis will be covered.  

Daniel Needleman

Further involvements