Microscopy Conference 2023

Focus on the 3D architecture of mitotic spindles in human cells

LS 3: Imaging of large volumes and plastic section tomography

Throughout the last two decades different volume scanning electron microscopy (vEM) methods have gained more importance in the life sciences allowing new questions to be answered for samples from cells to organisms and tissues. New developments for hardware and software for transmission as well as scanning electron microscopy have paved the road to new exciting research topics to be addressed. This session will illustrate the diversity of methods and applications of the current volume SEM modalities, namely array tomography (AT), serial block face SEM (SBEM) and focused ion beam SEM (FIB-SEM), as well as plastic tomography focusing on higher resolution. Besides different research topics, the challenges linked to the sample preparation, imaging and data analysis will be covered.  

Imaging of large volumes and plastic section tomography

Invited talk

poster session 8

Poster session LS 5: Correlative and multimodal microscopy

Poster presentations

Session chairs

Correlative microscopy aims at a combination of different imaging modalities to analyze a single specimen by more than one microscopic technique. Recent developments in microscopy as well as in computational approaches make it more and more feasible to combine different imaging modalities in a correlative and multimodal way. Therefore, this session will focus not only on the ‘classic combination’ of light and electron microscopy but will cover also other approaches that include X-ray, Raman- or atomic force microscopy (AFM). It is also aimed to report on new computational approaches, such as machine learning to analyze the obtained CLEM data.

copernicum

Correlative and multimodal microscopy

Life sciences

Prof.

Thomas Müller-Reichert

Engagements

28/02/2023

01/03/2023