Microscopy Conference 2023

It is not easy being green: the technical path for the ultrastructural study of the locomotory apparatus of the moss <em>Physcomitrium patens</em>

LS 3: Imaging of large volumes and plastic section tomography

Throughout the last two decades different volume scanning electron microscopy (vEM) methods have gained more importance in the life sciences allowing new questions to be answered for samples from cells to organisms and tissues. New developments for hardware and software for transmission as well as scanning electron microscopy have paved the road to new exciting research topics to be addressed. This session will illustrate the diversity of methods and applications of the current volume SEM modalities, namely array tomography (AT), serial block face SEM (SBEM) and focused ion beam SEM (FIB-SEM), as well as plastic tomography focusing on higher resolution. Besides different research topics, the challenges linked to the sample preparation, imaging and data analysis will be covered.  

Ana Laura Sousa

Engagements

28/02/2023