Microscopy Conference 2023

Cryo-electron tomography of SARS-CoV-2 replication and assembly sites reconstituted in cells

LS 2: Cryo-tomography

Cryo-electron microscopy (cryoEM) and cryo-electron tomography (cryoET) provide us a high-resolution gateway to the molecular processes that underlie life. These techniques allow us to view biological molecules in their native state and at high resolution, to image and determine their dynamics and, moreover, their socio-molecular interactions. Still, improvements and developments in sample preparation, workflows that integrate different tools and co-localization strategies, along with new image processing approaches are needed to exploit the full potential of these methods. This session will discuss the latest technological advances and present tangible examples that demonstrate the strength of both cryoEM and cryoET.

Cryo-electron microscopy and cryo-electron tomography

Abstract talk

LS 6: Pathology, pathogens and diagnostics

Characterization of vRNP clustering in influenza A virus-infected cells using in-situ cryo-electron tomography

Zooming into the spread and entry of influenza A viruses by correlative light and electron microscopy

Microscopy helps to diagnose and to understand diseases. The session will cover all applications of electron microscopy in this field, from high-resolution work by cryo-electron microscopy to routine diagnostic electron microscopy using thin section or negative staining electron microscopy.

Pathology, pathogens and diagnostics

Invited talk

copernicum

Life sciences

Petr Chlanda

Speaker

Further involvements