Despite their importance in the induction and maintenance of the blood-brain barrier (BBB) main features throughout life, brain pericyte physiology is understudied as well as their fate under neuropatholigical conditions, i.e. once exposed to pro-inflammaroty cytokines produced by brain cells under neurodegenaritive processes. This presentation aims (i) to characterize brain pericyte physiological features and the proteimic switch of this cell type induced by BBB endothelial cells, and (ii) to highlight the pathological events in brain pericytes leading to a disease propagation in autocrine and paracrine ways. All the data have been generated through mass spectrometry approaches (MS): unlabelled quantitative Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH)-MS and Tandem Mass Tag (TMT)-MS. Alltoghether, these data suggest a proteomic switch of brain pericyte to aswer to BBB endothelial cells needs, and confort the role of cytokines-mediated inflammaroty processes under neurodegenerative disorders since brain pericyte is the first cell type of the neurovascular unit to undergo stress conditions and is able to diffuse it in its neighborhood. This study confirms the key role of brain pericyte in BBB homeostasis in physiological and pathological contexts.