Hypoxic Storage of Leucodepleted Erythrocyte Concentrates in PAGGS-M Results in Increased Product Quality over Conventional Products as Assessed by Adenosine Triphosphate and 2,3-Diphosphoglycerate

Storage of Erythrocyte Concentrates (EC) under hypoxia has been made technically possible under GMP. It has been shown to preserve red blood cell (RBC) function, resulting in increased oxygen uptake as well as oxygen release, and extended in vivo survival of transfused RBC. We here aimed to study RBS quality in ECs derived from normal whole blood donations.

ECs from 500ml whole blood donations were prepared by the buffy coat method, stored in PAGGS-M solution and de-oxygenated within 24h by flow through CE-marked HemaNext-ONE deoxygenation system (Hemanext, Lexington, MA, USA). They were subsequently stored in oxygen-impermeable bags at 2-6 degrees C over 42 days, and assessed for quality parameters including Adenosin Triphosphate (ATP) and 2,3-Diphosphoglycerate (2,3-DPG).

Hypoxia was efficiently reached and maintained between 14 and 17% over the storage time of 42 days. Mean 2,3-DPG levels in deoxygenated RBC amounted to 12 to 16 µmol/g hemoglobin (Hb) between day 0 and day 21 of storage, 6 µmol/g Hb at day 28 and 2 µmol/g Hb at the end of storage, whereas they approached zero values already by day 14 in conventionally stored products. ATP levels were above 4 µmol/g Hb between days 0 and 28 and declined to 3.5 µmol/g Hb at the end of storage at day 42, and were continuously higher compared to conventional RBCs. All other parameters (Hb content, hematocrit, hemloysis) remained within the range of conventional controls.

Hypoxic storage of RBSs resulted in significant improvements in red cell strorage quality parameters ATP and 2,3-DPG. Hypoxia-stored RBCs met guideline and license requirements. These findings may also have implications on establishing improved storage conditions of RBCs in Di(2-ethylhexyl)terephthalate (DEHT)-free blood bag systems.

Es wurden keine Interessenkonflikte bekannt.