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56. Jahrestagung der Deutschen Gesellschaft für Transfusionsmedizin und Immunhämatologie

PD Dr.

Vorsitz

Prof. Dr.

Neue Richtlinien Hämotherapie 2023

eingeladene Redner

Vortrag

Leitlinien supportive Therapie:  Anämie

Leitlinie Polytrauma

Beispiele zum Patient-Blood-Management an einer Uniklinik

Prof.

Counting of transfused platelets in recipient blood by digital PCR of 7 mitochondrial markers – analytical qualities

Quality Management

Background Recovery and survival of transfused platelets can be assessed by digital PCR of mitochondrial markers. Therefore, we investigated the key analytical qualities of the droplet digital PCR of seven mitochondrial markers. Methods The SPEF1 gene served as internal control for the entire process and was tested for intra-and inter-assay variability with 20 replicates and 10 independent PCR runs, respectively. Limit of blank (LoB: mean copy number NTC + 1.645 x SD copy number NTC), limit of detection (LoD: LOB + 1.645 x SD copy number non-blank material) and limit of quantification (LoQ: lowest platelet concentration with a coefficient of variation ≤ 25%) were determined with 10different PCR runs and samples measured in duplicate. The linearity was evaluated by dilution of platelets (0.07 to 10 G/L) tested in quadruples. Determination of the assays" validity was done by comparison with flow-cytometry and hematology analyzers (Symex XS 800i and Advia 2120i).Results Assay linearity was demonstrated by R^2-values above 0.99, the slope varied in respect to the different markers between 0.738 and 1.382 and intercept ranged from -0.03 to 0.16. DNA was prepared from EDTA anticoagulated samples (n=10) on a daily basis and tested in the ddPCR assay. The maximal difference in platelet count between day one and day five of sample preparation was 0.5 G/L. DNA samples stored at -30°C showed mean differences to the initial results of 0.131± 0.5 (one month), 0.121 ± 0.5 (three months), 0.130 ± 0.2 (six month) and 0.126 ± 0.180 G/L platelets after 12 months" storage. Validity of ddPCR results was in the range of R^2 = 9.3 – 9.5. The calculated recovery of the internal control was 79.9%±7.93.Conclusion Quantification of endogenous and transfused platelets with ddPCR offers a sensitive and reliable tool to monitor platelets in patients.Offenlegung Interessenkonflikt: Kein InteressenkonfliktInvited talks abstract/summary

Freier Vortrag

Long-COVID patients show enhanced formation of COHb and changes in Raman spectra of Hemoglobin – associated vibrational modes

COVID-19

Background Background: The Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was the cause of the global COVID-19 pandemic. Irrespective of the disease severity, a growing number of patients report a delayed state of exhaustion called chronic fatigue syndrome (CFS). At least 10% of all COVID-19 patients experience various persistent or new health complaints after SARS-CoV-2 infection lasting longer than three months. In addition to CFS, Long-COVID patients also suffer from dyspnea, anemia andMethods others. Recently, we demonstrated that SARS-CoV-2-induced dysregulation in hemoglobin (Hb)- and iron-metabolism contributes to the severe systemic course of COVID-19. Changes in Hb structure may also be significantly involved in the development of Long-COVID symptoms. Therefore, we analyzed Hb- and iron-metabolism in Long-COVID-Patients. Methods: We performed blood gas analysis of more than 20 Long-Covid-19 and control patients. Furthermore, we measured hematological parameters and Raman spectra of red blood cells (RBCs) from peripheral blood of Long-COVID patients and compared those with healthy donors. Results Results: Whereas, no differences in the RBC count, Hb content and hematocrit were observed in the blood of Long-COVID patients compared to healthy donors, the amount of COHb was significantly increased. Furthermore, we found significant changes in spin state of the iron in Hb, which were partially comparable to packed RBCs stored for 42 days. In addition, BE- and BI values but not pH were diminished in Long-COVID patients compared to healthy donors, albeit still within the normal range. Lactate levels were also significantly elevated in the blood of Long-COVID patients. Thus, O2Hb was reduced in Long-COVID patients.Conclusion Conclusions: Our data shows drastic changes in Hemoglobin–associated vibrational modes in samples of Long-COVID patients. Together with the diminished Acid-base balance and enhance formation of COHb, we suggest an impaired erythrocyte functionality in Long-COVID patients, leading to diminished oxygen supply. This in turn could be an explanation for the CFS and dyspnea as well as anemia. The project was funded by the Roland-Ernst-Stiftung.Offenlegung Interessenkonflikt: Wir haben keine InteressenkonflikteInvited talks abstract/summary

MOA 01+02

Sektion Hämotherapie

Sektionssitzungen

Sektion Hämotherapie

Appointment

Vorsitz

Programme