Jacqueline Hausherr (Essen/ DE), Dr. Miriam Cantore (Essen/ DE), Fabian Nocke (Essen/ DE), Prof. Dr. Katja Bettina Ferenz (Essen/ DE), Dr. Nikolaus Pizanis (Essen/ DE), Omar Abou-Issa (Essen/ DE), Prof. Markus Kamler (Essen/ DE)
Background
Normothermic machine-perfusion (NT-MP) for organ preservation still depends on perfusates spiked with erythrocytes and thus is limited by donor blood shortage. Perfluorodecalin-based albumin-derived artificial oxygen carriers (A-AOC) display a promising blood alternative. In vitro experiments in cardiomyocytes confirmed the A-AOCs' ability to attenuate ischemia-elicited damage. The aim of this project was to establish a physiological model in which A-AOCs can be tested as blood surrogate.
Methods
NT-MP was chosen as physiological model for testing the functionality of A-AOCs in isolated perfused porcine hearts. Parameters such as oxygen partial pressure, pH-value, level of electrolytes and metabolic parameters of the perfusate were studied by a blood gas analyzer (BGA, ABL 815) and photometry (respons 920).
Results
After first pilot experiments, the perfusion system was successfully built with a heater, a perfusate reservoir, an oxygenator as well as pH- and pO2-sensors. Preliminary experiments in the established perfusion apparatus showed that the perfusion with a preservation solution was able to maintain a contracting heart for at least one hour. Furthermore, the level of Ca2+, K+, Na+ and lactate as well as oxygen, pH, lactate dehydrogenase and aspartate aminotransferase were successfully monitored throughout the perfusion experiments
Conclusion
After successful establishment of the experimental set-up for perfusing porcine hearts, we can investigate the A-AOCs' potential to reduce ischemia-reperfusion injury (IRI) in an ex vivo heart perfusion. Parameters for IRI (i.g. apoptosis, oxidative stress, Troponin I) will be investigated in the future. Our group previously demonstrated a successful application of A‑AOCs in a Langendorff-perfused rat heart. Therefore, an overall improvement of organ viability is expected for NT-MP with A-AOCs
Offenlegung Interessenkonflikt:
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