Die Rolle der Prokaryoten in chronisch infiziertem oder entzündetem Gewebe

Introduction

As part of the microbiome, different species of prokaryotes reside in the biotope of the human body. Some of these species or specific subtypes are able to alter the physical health in the form of chronic infections and inflammations. A better characterization of their interaction with the human tissue in terms of their localization and innate virulence factors would improve the understanding of etiologies driven by prokaryotes in their host.

Materials/Methods

Prokaryotes cultivated from swabs or infected tissue were used to characterize their composition and cellular properties. These species were also used to determine the specificity of IR-fluorescent probes able to localize the prokaryotes in biofilms. A genomic approach was applied to give insight into virulence factors altering the pathogenic properties of the prokaryotes.

Results

The general microbiome composition differed between healthy and chronically infected tissue. The fluorescent probes showed good specificity to see targeted prokaryotes. The IR signal could be easily discriminated from the autofluorescence common in tissue. By this means, the prokaryotes could also be localized in tissue samples. Species isolated from chronically infected tissue contained several genes connected to prokaryote virulence.

Discussion

The differences in virulence give insight into the source of inflammation caused by the microbiome. The localization of the bacteria in tissue could be used to facilitate surgery for biofilm removal from chronically infected tissue.

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