7th joint conference "Joint Microbiology & Infection Conference 2024" of the DGHM and VAAM

Prof. Dr.

Chair

Introduction EKC Würzburg-Mwanza

Others

Key note I: Africa and its microbes – a continent in transition

Invited Speaker

Invited Talk

Key note II: The long road to development of new drugs and treatments in filariasis

Professor

Effectiveness of a timed praziquantel treatment of schoolchildren in relation to seasonal transmission of urogenital schistosomiasis in North Western Tanzania

Oral Presentation

PD. Dr.

Genetic Characteristics, Virulence Factors, and Clonality of ESBL-Producing E. coli and K. pneumoniae: a Comparative Study During and After National Action Plan on Antimicrobial Resistance in Mwanza, Tanzania.

Diagnostic and Clinical Microbiology

Introduction: Antimicrobial resistance (AMR) poses a significant global health threat, necessitating regional-specific continuous surveillance to understand its dynamics and implications.Goals: To respond to the changing landscape of AMR, a cross-sectional hospital-based study was conducted in two periods: June 2019 – June 2020, during the first Tanzania National Action Plan on AMR (NAP-AMR), and March – July 2023, after its completion.Materials and Methods: Patients&quot; sociodemographic and clinical data were collected by pre-tested structured questionnaires. A total of 2648 and 1929 samples (blood, urine, and pus) collected during and after NAP-AMR respectively, were cultured to isolate bacterial pathogens; identification was based on VITEK MS and 16S rDNA Sanger sequencing, and VITEK 2 was used to determine susceptibility towards antibiotic agents. Next-generation sequencing on the Illumina NextSeq 550 platform was used for sequencing ESBL E. coli (n=22) and ESBL K. pneumoniae (n=23) out of 64 and 79 ESBL strains, respectively isolated during NAP-AMR.Results: ESBL-producing E. coli and K. pneumoniae phenotypes increased significantly after NAP-AMR [53.4%(134/268) vs. 73.8%(175/237), p&lt;0.0001]. Specific increase for ESBL-E. coli [41.3%(64/155) vs. 61.7%(74/120), p=0.0084] and ESBL-K. pneumoniae [69.9%(79/113) vs. 85.5%(100/117), p=0.0057] was remarkable. E. coli ST 648 (40.9%, 9/22) and K. pneumoniae ST 280 (23.1%, 6/23) were predominant. The IncFII plasmid-replicon was predominantly found in both E. coli (68.2%, 15/22) and K. pneumoniae (65.2%, 15/23). The blaCTX-M-15 gene was found in 81.5% (18/22) E. coli and all 23 K. pneumoniae. The virulence genes yfcV, fyuA, sitA, and traT were found in 68.2%, 72.7%, 72.7%, and 77.3% of E. coli respectively while the virulence genes acrA/B, entA/B/C/E, fepA-D/G, fimA-I/K, and mrkA-D/F/H-J were found in all K. pneumoniae.Summary: This study underscores the escalating threat of highly virulent strains of ESBL-producing E. coli and K. pneumoniae causing infections. The study advocates for the stringent implementation of IPC measures to curb the spread of antibiotic-resistant pathogens and associated public health risks.

Closing remarks

Salon Beatrix

SFB Session

Microbiology in Africa Session (with Else-Kröner Center)

SFB Session
Microbiology in Africa Session (with Else-Kröner Center)

Appointment

Chair

Programme

SFB SessionMicrobiology in Africa Session (with Else-Kröner Center)

Appointment

Chair

Programme

SFB Session
Microbiology in Africa Session (with Else-Kröner Center)