Streptavidin-biotin interaction: Basis for surface modification of implant surfaces

Abstract text (incl. figure legends and references)

Implants are used to replace damaged tissue or missing body parts. Concerning on biofilm formation of bacteria around the implant, the risk of an infection is given. To counteract this, surfaces can be biofunctionalized. The use of a biotin derivative and streptavidin to produce antibacterial surfaces has shown positive effects. This study builds on preliminary work on biofunctionalization of surfaces in our biophysics group [1] [2]. The molecular layer systems were studied with surface plasmon resonance spectroscopy and contact angle measurements. Kinetic measurements showed that on a biotinylated surface, the adsorbed streptavidin remains molecularly intact bound than on a desthiobiotinylated surface (Fig. 1). Furthermore, medical implants must be stored appropriately to ensure maximum biofunctionalization efficiency. A phosphate-buffered saline solution provides a suitable storage medium, as the progression of the contact angle remains as well as surface plasmon resonance reasonably constant (Fig. 2) [3].

References

[1] Volker Ettelt, Katharina Ekat, Peer W. Kämmerer, Bernd Kreikemeyer, Matthias Epple, and Michael Veith. Streptavidin-coated surfaces suppress bacterial colonization by inhibiting non-specific protein adsorption. Journal of biomedical materials research. Part A, 106(3):758–768, 2018.

[2] Michael Lehnert, Miriam Gorbahn, Marcus Klein, Bilal Al-Nawas, Ingo Köper, Wolfgang Knoll, and Michael Veith. Streptavidin-coated TiO2 surfaces are biologically inert: protein adsorption and osteoblast adhesion studies. Journal of biomedical materials research. Part A, 100(2):388–395, 2012.

[3] Chaymae Boukari. Optimierung von biofunktionalisierten Oberfläachen und Anwendungen in der Biotechnologie. Bachelorarbeit, Westfälische Hochschule, Recklinghausen, 31.08.2021.