Nelly Senze Nnane (Braunschweig, DE), Sushobhan Sarker (Braunschweig, DE), Dr. Carina Mikolai (Hannover, DE), Dr. Andreas Winkel (Hannover, DE), Prof. Dr. Meike Stiesch (Hannover, DE), Prof. Dr. Dagmar Wirth (Braunschweig, DE), Prof. Dr. Henning Menzel (Braunschweig, DE)
Abstract text (incl. figure legends and references)
Introduction
Replacing unwanted or lost teeth with osseointegrated implants has shown significant advancement, with more than 89% success rate. However, bacterial infections from biofilm formation leading to peri-implantitis can result in the undesired loss of implants. Due to the resistance of bacteria in the biofilm against antimicrobial agents, several kinds of research have been conducted to prevent the invasion of implant surrounding by bacteria, such as antibacterial coatings.
Objective
To address biocompatibility issues of our drug delivery system currently under development, this project aims to fabricate a polymer coating containing all the essential elements to study it in terms of biocompatibility, biodegradability, cell viability and, if present, antimicrobial activity in the 3D-INTERbACT in vitro model.
Materials & methods
In 5 synthetic steps, raw chitosan was purified, acetylated and functionalized with maleimide (Cs-Ml). CS-Ml was reacted with a furan-modified ciprofloxacin derivative, yielding chitosan with ciprofloxacin side chains (s. Fig.1). This synthesis was confirmed by size exclusion chromatography, 1H-NMR, IR.
Results
The Ciprofloxacin derivatized chitosan was characterized regarding the degree of substitution by 1H-NMR. We observed solubility changes upon introduction of the ciprofloxacin, see Figure 2A. At higher degrees of substitution, gel formation could be observed, see Figure 2B.
Conclusion
Ciprofloxacin containing chitosan was prepared with different degrees of substitution, which differ in their solubility in aqueous solutions. The polymers will be applied as coatings on titanium surfaces and used in in-vitro investigations.