Dikshita Madkatte (Heilbad Heiligenstadt, DE), Marcus Soter (Heilbad Heiligenstadt, DE), Gurunath Apte (Heilbad Heiligenstadt, DE), Dr. Thi-Huong Nguyen (Heilbad Heiligenstadt, DE; Ilmenau, DE)
Abstract text (incl. figure legends and references)
Introduction:
Platelets have a strong tendency to be activated immediately when contacting any non-physiological surfaces. The development of surfaces for anti-platelet activation is important not only for platelet storage bags/tubing but also for many blood-contacting devices. The present bag products for platelets currently have a limited storage duration of only a few days. Since only less than 1% of donated blood is platelets, the availability and storage of these products are of particular interest in the medical field. To date, no satisfied surfaces that allow strongly inhibiting platelet activation have been developed.
Objectives:
We use biocompatible protein-based materials such as modified collagen and gelatin to create 2 & 2.5D Micro-/ nanostructured surfaces to reduce platelet-surface activation.
Material and Methods:
We synthesize protein-based materials and cross-linked by photo-polymerization. Biomaterial compositions based on extracellular matrix (ECM) proteins are used to modify the surface. To create Micro-/ nanostructures (several tenths of a µm down to the lower 400 nm range) fluid force microscopy (Fluid FM) is used. The printed structures are investigated using AFM and SEM imaging, contact angle measurement, and the platelet de-adhesion force is measured using Fluid FM.
Results:
Our newly synthesized materials are printable. A change in wettability and roughness of the surface properties can be observed. Further, the modification of the surface influences the behavior of platelet-surface activation Furthermore; structured surfaces significantly influence the response of platelets.
Conclusion:
The different structures, biomaterials, surface stiffness, and adhesion forces have an obvious impact on the response of platelets.