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Poster
P 29

Antibacterial properties of functionalized silk fibroin and sericin membranes for wound healing applications in oral and maxillofacial surgery

Termin

Datum: 14.09.2023

Zeit: 18:45 – 18:45

Redezeit: 0 Min.

Diskussionszeit: 0 Min.

Ort / Stream:

Foyer

Session

Poster Exhibition

Themen

Antimicrobial coatings
Surface modification technologies

Mitwirkende

Sogand Schäfer (Hamburg, DE), Prof. Dr. Dr. Ralf Smeets (Hamburg, DE), Marius Köpf (Aachen, DE), Aleksander Drinic (Aachen, DE), Alexander Kopp (Aachen, DE), Nadja Kröger (Köln, DE), Philip Hartjen (Hamburg, DE), Alexandre Assaf (Hamburg, DE), Farzaneh Aavani (Hamburg, DE), Thomas Beikler (Hamburg, DE), Ulrike Peters (Hamburg, DE), Imke Fiedler (Hamburg, DE), Björn Busse (Hamburg, DE), Ewa Stürmer (Hamburg, DE), Tobias Vollkommer (Hamburg, DE), Prof. Dr. Dr. Martin Gosau (Hamburg, DE), Sandra Fuest (Hamburg, DE)

Abstract

Abstract text (incl. figure legends and references)

Oral wounds are among the most troublesome injuries which easily affect the patients' quality of life. To date, the development of functional antibacterial dressings for oral wound healing remains a challenge. In this regard, we investigated antibacterial silk protein-based membranes for the application as wound dressings in oral and maxillofacial surgery. The present study includes five variants of casted membranes, i.e., i) membranes-silver nanoparticles (CM-Ag), ii) membranes-gentamicin (CM-G), iii) membranes-control (without functionalization) (CM-C), iv) membranes-silk sericin control (CM-SSC), and v) membranes-silk fibroin/silk sericin (CM-SF/SS), and three variants of nonwovens, i.e., i) silver nanoparticles (NW-Ag), ii) gentamicin (NW-G), iii) control (without functionalization) (NW-C). The surface structure of the samples was visualized with scanning electron microscopy. In addition, antibacterial testing was accomplished using agar diffusion assay, colony forming unit (CFU) analysis, and qrt-PCR. Following antibacterial assays, biocompatibility was evaluated by cell proliferation assay (XTT), cytotoxicity assay (LDH), and live-dead assay on L929 mouse fibroblasts. Findings indicated significantly lower bacterial colony growth and DNA counts for CM-Ag with a reduction of bacterial counts by 3log levels (99.9% reduction) in CFU and qrt-PCR assay compared to untreated control membranes (CM-C and CM-SSC) and membranes functionalized with gentamicin (CM-G and NW-G) (p < 0.001). Similarly, NW-G yielded significantly lower DNA and colony growth counts compared to NW-Ag and NW-C (p < 0.001). In conclusion, CM-Ag represented 1log level better antibacterial activity compared to NW-G, whereas NW-G showed better cytocompatibility for L929 cells. As data suggest, these two membranes have the potential of application in the field of bacteria-free oral wound healing.